Chemical deprotection strategy for the elaboration of misacylated transfer RNA's

摘要

In recent years, the elaboration of proteins containing synthetic amino acids at predetermined sites has become technically feasible; the strategy employed involves readthrough of nonsense codons with misacylated sup- pressor tRNA's As first shown by Hecht and co- workers, misacylated tRNA's (Scheme 1) are accessible by T4 RNA ligase-mediated coupling of aminoacylated pCpA or pdCa derivatives with tRNA's from which the 3'-terminal dinucleotide has been removed.