A New Method for Conjugation of Carbohydrates to Proteins Using an Aminooxy-Thiol Heterobifunctional Linker

摘要

A new,efficient,and mild protocol is presented for the coupling of saccharides to proteins.First,a heterobifunc-tional aminooxy-thiol linker is coupled to the bromoacylated protein to introduce aminooxy groups through thioether linkages.Condensation of the aminooxylated protein and aldehydo/keto-derivatized carbohydrates affords covalent saccharide-protein constructs.Uncoupled saccharide can be recovered in its original form.The scope of our protocol is exemplified by the coupling of neutral mono- and tetrasac-charides and a negatively charged ribitol-phosphate construct to BSA.