Construction of a human Bax eukaryotic expression vector and its ex- pression in human drug resistant gastric cancer cell line SGC7901/ VCR

摘要

Objective: To construct a human Bax eukaryotic expression vector and detect its expression in hu- man drug resistant gastric cancer cells. Methods: Bax gene was first inserted into polyclonal sites of pBK- CMV vector. Then the recombinant plasmid vector was transfected into vincristine resistant human gastric Cancer cells SGC7901/VCR by lipofectamine. G418 was used to select resistant clones. Lastly, Western blot Was used to observe the expression of Bax protein.