Research Note: A Multiplex Reverse Transcription Polymerase Chain Reaction Method for the Detection of Foodborne Viruses

摘要

A multiplex reverse transcription polymerase chain reaction(RT-PCR) method was developed for the simultaneous de- tection of the human enteroiruses, hepatitis A virus (HAV) and Norwalk virus (NV). Poliovirus type 1 (PV1) was chosen as a model for the human enterovirus group. Thee different sets of primers were used to produce three size-specific amplicons of 435 bp, 270 bp, and 192 bp for PV1, NV, and HAV, respectively. RT-PCR products were separated by agarose gel electrophoresis, and amplicon identity was confirmed by Southern transfer followed by DNA hybridization using nonradioac- tive, digoxigenin-labeled internal probes.