Nucleic Acid Persistence in Heat-Illed Escherichia coli O157:H7 from Contaminated Skim Milk

摘要

Polymerase chain reaction (PCR) and reverse transcriptase (RT)-PCR using primers targeting 16S rRNA sequences in Escherichia coli O157:H7 wee applied to monitor the stability of rDNA and rRNA in cells killed by mild heat treatment (60 deg. C) In skim milk. Serial dilutions of purified RNA and DNA from E. coli O157:H7 in skim milk were amplified by RT-PCR or PCR, Respectively, before heat treatment and at time points 0,6,12,24, and 48 h after heating.