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Research Note: Evaluation of a Fluorodensitometric Method for Analysis of Ergosterol as a Fungal Marker in Compound Feeds

机译:研究报告:荧光光度法分析麦角固醇作为复合饲料中真菌标记的分析

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摘要

Ergosterol is the principal sterol of fungi and plays an essential role as a component of the cell membrane and other cell constituents. This molecule is considered a good marker of fungalcontamination in foods and feeds. This paper reports a rapid and sensitive method to test ergosterol content in compound feeds based n fluorodensitometry after thin-layer chro- matography (TLC) separation. This method involves a thermal treatment of TLC plates that leads to the formation of a highly fluorescent ergosterol derivative.
机译:麦角固醇是真菌的主要固醇,在细胞膜和其他细胞成分中起着至关重要的作用。该分子被认为是食品和饲料中真菌污染的良好标志。本文报道了一种快速灵敏的方法,用于在薄层色谱法(TLC)分离后,通过n荧光光度法测定复合饲料中的麦角固醇含量。该方法涉及对TLC板的热处理,导致形成高荧光麦角固醇衍生物。

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