Researhc Note: An Oligonucleotide-Ligation Assay for the Differentiation Between Cyclospora and Eimeria spp. Polymerase Chain Reaction Amplification Products.

摘要

An oligonucleotide-ligation assay (OLA) was developed and compared to a restriction fragment length polymrphism (RFLP) test for distinguishing between 294-bp polymerase chain reaction (PCR) amplification products of the 18S rRNA gene from Cyclospora and Eimeria spp. The PCR/OLA correctly distinguished between there Cyclospora, three E. Tenella, and one E. mitis strains and the ratio of positive to negative spectrophotometric absorbance (A_490) values for each strain ranged form 4.086 to 15.280 (median 9.5). PCR/OLA provides a rapid, reliable, spectrophotometric alternative to PCR/RFLP.