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Review: Limitations of Molecular Biological Techniques for Assessing The Virological Safety of Foods

机译:综述:评估食品病毒学安全性的分子生物学技术的局限性

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enteric viruses, including hepatitis A, Norwalk, and Snow Mountain viruses, Hawaii agent, and rotaviruses have been associated with outbreaks of foodborne illness. Classical culturing procedures are available for poliovirus; however, hepatitis A. Noralk, and many of the other viruses and agents cannot be propagated in cell culture, therefore, molecular biological Tools have emerged as a possible means to detect enteric viruses in foods and environmental samples. There are limitations However in the application of polymerase chain reaction and reverse transcription polymerase chain reaction that restrict their Useufulness for measuring the virological safety of foods.
机译:肠道病毒,包括甲型肝炎病毒,诺沃克病毒和雪山病毒,夏威夷病毒和轮状病毒,与食源性疾病的爆发有关。脊髓灰质炎病毒有经典的培养程序。但是,甲型肝炎病毒,诺拉克病毒以及许多其他病毒和媒介无法在细胞培养中繁殖,因此,分子生物学工具已成为检测食品和环境样品中肠病毒的一种可能手段。然而,在聚合酶链反应和逆转录聚合酶链反应的应用中存在局限性,这限制了它们在测量食品的病毒学安全性方面的用途。

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