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High-Salt Preadaptation of Vibrio parahaemolyticus Enhances Survival in Response to Lethal Environmental Stresses

机译:副溶血性弧菌的高盐重适应可增强对致命环境压力的存活

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摘要

Adaptation to changing environmental conditions is an important strategy for survival of foodborne bacterial pathogens. Vibrio parahaemolyticus is a gram-negative seafoodborne enteric pathogen found in the marine environment both free living and associated with oysters. This pathogen is a moderate halophile, with optimal growth at 3% NaCl. Among the several stresses imposed upon enteric bacteria, acid stress is perhaps one of the most important. V. parahaemolyticus has a lysine decarboxylase system responsible for decarboxylation of lysine to the basic product cadaverine, an important acid stress response system in bacteria. Preadaptation to mild acid conditions, i.e., the acid tolerance response, enhances survival under lethal acid conditions. Because of the variety of conditions encountered by V. parahaemolyticus in the marine environment and in oyster postharvest facilities, we examined the nature of the V. parahaemolyticus acid tolerance response under high-salinity conditions. Short preadaptation to a 6% salt concentration increased survival of the wild-type strain but not that of a cadA mutant under lethal acid conditions. However, prolonged exposure to high salinity (16 h) increased survival of both the wild-type and the cadA mutant strains. This phenotype was not dependent on the stress response sigma factor RpoS. Although this preadaptation response is much more pronounced in V. parahaemolyticus, this characteristic is not limited to this species. Both Vibrio cholerae and Vibrio vulnificus also survive better under lethal acid stress conditions when preadapted to high-salinity conditions. High salt both protected the organism against acid stress and increased survival under -20℃ cold stress conditions. High-salt adaptation of V. parahaemolyticus strains significantly increases survival under environmental stresses that would otherwise be lethal to these bacteria.
机译:适应不断变化的环境条件是食源性细菌病原体生存的重要策略。副溶血性弧菌是革兰氏阴性的海鲜传播的肠道病原体,存在于海洋环境中,自由生活并伴有牡蛎。该病原体是中等嗜盐菌,在3%NaCl下具有最佳生长。在施加于肠细菌的多种压力中,酸性压力可能是最重要的压力之一。副溶血弧菌具有赖氨酸脱羧酶系统,负责赖氨酸脱羧成碱性产物尸胺,这是细菌中重要的酸胁迫响应系统。重新适应温和的酸性条件,即耐酸反应,可以提高在致命酸性条件下的存活率。由于副溶血弧菌在海洋环境和牡蛎捕捞后设施中遇到的条件多种多样,因此我们研究了高盐度条件下副溶血弧菌对酸的耐受性。短期预适应盐浓度为6%可以提高野生型菌株的存活率,但在致死酸性条件下不能提高cadA突变体的存活率。但是,长时间暴露于高盐度(16小时)可提高野生型和cadA突变株的存活率。该表型不依赖于应激反应σ因子RpoS。尽管这种预适应性反应在副溶血性弧菌中更为明显,但该特征并不限于该物种。当预先适应高盐度条件时,霍乱弧菌和创伤弧菌在致命的酸性胁迫条件下也能更好地生存。高盐既能保护生物体免受酸胁迫,又能在-20℃冷胁迫条件下提高生存率。副溶血弧菌菌株的高盐适应性显着提高了在环境胁迫下的生存,否则这些环境将使这些细菌致死。

著录项

  • 来源
    《Journal of food protection》 |2014年第2期|246-253|共8页
  • 作者单位

    Department of Biological Sciences, University of Delaware, Newark, Delaware 19716, USA;

    Department of Biological Sciences, University of Delaware, Newark, Delaware 19716, USA;

    Department of Biological Sciences, University of Delaware, Newark, Delaware 19716, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
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  • 入库时间 2022-08-17 23:25:11

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