首页> 外文期刊>Journal of food protection >Gene Transcription Patterns of pH- and Salt-Stressed Listeria monocytogenes Cells in Simulated Gastric and Pancreatic Conditions
【24h】

Gene Transcription Patterns of pH- and Salt-Stressed Listeria monocytogenes Cells in Simulated Gastric and Pancreatic Conditions

机译:在模拟的胃和胰腺条件下,pH和盐胁迫的单核细胞增生李斯特菌细胞的基因转录模式

获取原文
获取原文并翻译 | 示例
       

摘要

A Listeria monocytogenes subgenomic array, targeting 54 genes involved in the adhesion, adaptation, intracellular life cycle, invasion, and regulation of the infection cycle was used to investigate the gene expression patterns of acid- and salt-stressed Listeria cells after exposure to conditions similar to those in gastric and pancreatic fluids. Three L. monocytogenes strains, one laboratory reference strain (EGDe) and two food isolates (wild strain 12 isolated from milk and wild strain 3 isolated from fermented sausage), were used during the studies. Differences in the expressed genes were observed between the gastric and pancreatic treatments and also between the serotypes. Increased transcripts were observed of the genes belonging to the adaptation and regulation group for serotype 4b (strain 12) and to the invasion and regulation group for serotype 1/2a (strain EGDe). Interestingly, no significantly differentially expressed genes were found for serotype 3c (strain 3) in most cases. The genes related to adaptation (serotype 1/2a) and to intracellular life cycle and invasion (serotype 4b) were down-regulated in order to cope with the hostile environment of the gastric and pancreatic fluids. These findings may provide experimental evidence for the dominance of serotypes 1/2a and 4b in clinical cases of listeriosis and for the sporadic occurrence of serotype 3c.
机译:使用李斯特菌李斯特菌亚基因组阵列,针对与黏附,适应,细胞内生命周期,侵袭和感染周期调节有关的54个基因,研究了在类似条件下暴露于酸和盐的李斯特菌细胞的基因表达模式。那些在胃和胰液中。在研究过程中,使用了三株单核细胞增生李斯特菌菌株,一个实验室参考菌株(EGDe)和两个食品分离株(从牛奶中分离出的野生菌株12和从发酵香肠中分离出的野生菌株3)。在胃和胰腺治疗之间以及血清型之间观察到表达基因的差异。观察到属于血清型4b的适应和调控组(12株)和属于血清型1 / 2a的入侵和调控组(EGDe株)的基因转录本增加。有趣的是,在大多数情况下,没有发现血清型3c(菌株3)的显着差异表达基因。下调与适应性相关的基因(血清型1 / 2a)以及与细胞内生命周期和侵袭相关的基因(血清型4b),以应对胃液和胰液的不利环境。这些发现可能为李斯特菌病临床病例中1 / 2a和4b血清型占优势以及3c血清型零星出现提供实验证据。

著录项

  • 来源
    《Journal of food protection》 |2014年第2期|254-261|共8页
  • 作者单位

    Universita di Torino, Dipartimento di Scienze Agrarie, Forestall e Alimentari, Via Leonardo da Vinci 44, Grugliasco, 10095 Turin, Italy,Agricultural University of Athens, Department of Food Science and Technology, Laboratory of Food Quality Control and Hygiene, lera Odos 75,11855 Athens, Greece;

    Universita di Torino, Dipartimento di Scienze Agrarie, Forestall e Alimentari, Via Leonardo da Vinci 44, Grugliasco, 10095 Turin, Italy;

    Universita di Torino, Dipartimento di Scienze Agrarie, Forestall e Alimentari, Via Leonardo da Vinci 44, Grugliasco, 10095 Turin, Italy;

    Universita di Torino, Dipartimento di Scienze Agrarie, Forestall e Alimentari, Via Leonardo da Vinci 44, Grugliasco, 10095 Turin, Italy;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

  • 入库时间 2022-08-17 23:25:11

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号