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Mycobacterium avium subsp. avium in Lymph Nodes and Diaphragms of Pigs from One Infected Herd in the Czech Republic

机译:鸟分枝杆菌亚种捷克共和国一名感染牛群的猪的淋巴结和膜片中的鸟卵

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摘要

This study was performed on 40 finished pigs from one herd naturally infected with Mycobacterium avium subsp. avium. The aim was to investigate the presence and amount of M. a. avium in samples of lymph nodes and diaphragm tissues collected during routine postmortem inspection using the triplex quantitative real time PCR (qPCR) method. We collected, in total, 107 samples: various lymph nodes affected by gross tuberculosis (TB)-like lesions from 17 pig carcasses, as well as samples of head and mesenteric lymph nodes from 23 carcasses without TB-like lesions. Samples of diaphragm tissues were collected from all carcasses. M. a. avium was detected in one or more tissue samples collected from half of the slaughtered pigs tested. Samples of diaphragm tissues of three pigs with detected TB-like lesions contained M. a. avium (10~2 to 10~3 cells per g of sample); the organism was not detected in diaphragm tissues from pigs without TB-like lesions. The qPCR method may be useful for quantification of M. a. avium in pigs for the purposes of foodborne risk assessment.
机译:这项研究是用一只自然感染鸟分枝杆菌亚群的40头猪制成的。阿维姆目的是调查M. a。的存在和数量。使用三重实时定量PCR(qPCR)方法在常规死后检查期间收集的淋巴结和隔膜组织样本中的鸟卵。我们总共收集了107个样本:从17个猪屠体中感染了受总结核样(TB)样病变影响的各种淋巴结,以及从23个无结核样病变的尸体中头和肠系膜淋巴结采样。从所有屠体收集diaphragm肌组织样品。嘛。从一半测试的屠宰猪收集的一种或多种组织样品中检测到鸟卵。检测出结核样病变的三头猪的diaphragm肌组织样本中含有M. a。禽(每克样品10〜2至10〜3个细胞);在没有结核样病变的猪的diaphragm肌组织中未检测到这种生物。 qPCR方法可能对玛氏酵母的定量有用。猪中的禽肉,用于食源性风险评估。

著录项

  • 来源
    《Journal of food protection》 |2014年第1期|141-144|共4页
  • 作者单位

    Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic;

    Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic;

    Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic;

    Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic;

    Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

  • 入库时间 2022-08-17 23:25:08

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