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Effect of Bacterial Cell-Free Supernatants on Infectivity of Norovirus Surrogates

机译:无细菌上清液对诺如病毒替代品感染性的影响

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摘要

Bacterial metabolic products were evaluated for inhibitory effects on viral propagation in cell culture. Cell-free supernatants (CFS) were prepared from growth of Enterococcus faecalis ATCC 19433, Pseudomonas fluorescens ATCC 13525, Escherichia coli 08, Staphylococcus epidermidis ATCC 12228, Bacillus subtilis 168, Bacillus coagulans 185A, B. coagulans 7050, Clostridium sporogenes PA3679, and a commercial probiotic mixture of Lactobacillus acidophilus, Lactobacillus rhamnosus, Bifidobacterium bifidum, Lactobacillus salivarius, and Streptococcus thermophilus in microbiological medium or milk. The inhibitory effects of CFS on the propagation of murine norovirus 1 and Tulane virus in RAW 264.7 and LLCMK2 cells, respectively, were evaluated in the continuous presence of CFS or after exposure of host cells to CFS. Slight inhibition of viral propagation was observed for murine norovirus and Tulane virus in the continuous presence of CFS of B. subtilis 168 and E. faecalis 19433, respectively. CFS cytotoxicity was also determined by microscopic examination. Virus persisted in the CFS that demonstrated cytotoxic effects, suggesting a lack of direct effect of CFS on virions. The viral propagation indicates a general lack of competitive inhibition by bacterial extracellular products and bears significance in understanding the persistence of virus in food and human systems shared by bacteria that are recognized for their colonization and competitive capabilities.
机译:评估细菌代谢产物对细胞培养中病毒繁殖的抑制作用。从粪肠球菌ATCC 19433,荧光假单胞菌ATCC 13525,大肠杆菌08,表皮葡萄球菌ATCC 12228,枯草芽孢杆菌168,凝结芽孢杆菌185A,凝结芽孢杆菌7050,梭菌芽孢杆菌7050和梭菌芽孢杆菌的生长制备无细胞上清液(CFS)。微生物培养基或牛奶中的嗜酸乳杆菌,鼠李糖乳杆菌,双歧双歧杆菌,唾液乳杆菌和嗜热链球菌的商业益生菌混合物。在连续存在CFS或将宿主细胞暴露于CFS后,评估了CFS对RAW 264.7和LLCMK2细胞中鼠诺如病毒1和杜兰病毒的增殖的抑制作用。在分别存在枯草芽孢杆菌168和粪肠球菌19433的CFS的情况下,观察到鼠诺如病毒和杜兰病毒对病毒繁殖的轻微抑制。还通过显微镜检查确定了CFS的细胞毒性。病毒在CFS中持续存在,表现出细胞毒性作用,表明CFS对病毒体缺乏直接作用。病毒繁殖表明细菌胞外产物普遍缺乏竞争性抑制作用,并且对于理解细菌在食物和人类系统中的病毒在细菌和细菌共享的持久性方面具有重要意义,这些细菌因其定殖和竞争能力而得到认可。

著录项

  • 来源
    《Journal of food protection》 |2014年第1期|145-149|共5页
  • 作者单位

    Department of Animal and Food Sciences, University of Delaware, 044 Townsend Hall, 531 South College Avenue, Newark, Delaware 19716-2150, USA;

    Department of Animal and Food Sciences, University of Delaware, 044 Townsend Hall, 531 South College Avenue, Newark, Delaware 19716-2150, USA;

    Department of Animal and Food Sciences, University of Delaware, 044 Townsend Hall, 531 South College Avenue, Newark, Delaware 19716-2150, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

  • 入库时间 2022-08-17 23:25:08

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