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Inactivation of Escherichia coli O157 Bacteriophages by Using a Mixture of Ferrous Sulfate and Tea Extract

机译:硫酸亚铁和茶提取物的混合物灭活大肠杆菌O157噬菌体

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摘要

Bacteriophages (phages) have been used for biocontrol of Escherichia coli O157 and other pathogenic bacteria in many different matrices and foods, but few studies have included inactivation of residual phages in culture medium before plating and enumeration of surviving host bacteria for the assessment of phage efficacy. This oversight may lead to overestimation of phage efficacy. The ability of virucidal solution containing a mixture of ferrous sulfate [iron(Ⅱ) sulfate, FeSO_4] and tea extract [Fe(Ⅱ)T] to inactivate residual T5-like, T1-like, T4-like, and rV5-like phages was assessed using E. coli O157 as the host. At concentrations of ≥10 mM FeSO_4, all phages were not detected after 20 min in a broth culture model. Compared with the virucidal solution-free samples (1 to 96% recovery), Fe(Ⅱ)T (10 mM FeSO_4 plus 15% tea extract) recovered a greater (P < 0.01) number of E. coli O157 from phage-treated broth culture (97 to 100% recovery) and beef samples (52 to 100% recovery). Moreover, with the addition of Fe(Ⅱ)T, the number of bacteria surviving after exposure to T5-like or T4-like phages was greater (P < 0.01) than that after exposure to T1-like or rV5-like phages. Consequently, use of a virucide for phage inactivation is recommended to improve the accuracy of evaluations of phage efficacy for biocontrol of E. coli O157.
机译:噬菌体(噬菌体)已用于许多不同基质和食品中的大肠杆菌O157和其他病原细菌的生物防治,但是很少有研究包括在铺板前灭活培养基中残留的噬菌体并计数存活的宿主细菌以评估噬菌体功效。这种疏忽可能导致对噬菌体功效的高估。含有硫酸亚铁[硫酸铁(Ⅱ),FeSO_4]和茶提取物[Fe(Ⅱ)T]的杀病毒溶液灭活残留的T5样,T1样,T4样和rV5样噬菌体的能力。使用大肠杆菌O157作为宿主评估大肠杆菌。在浓度≥10 mM FeSO_4的情况下,在肉汤培养模型中20分钟后未检测到所有噬菌体。与无杀伤溶液的样品相比(回收率为1%到96%),Fe(Ⅱ)T(10 mM FeSO_4加15%的茶提取物)从噬菌体处理过的肉汤中回收了更多(P <0.01)的大肠杆菌O157。培养(97%至100%的回收率)和牛肉样品(52%至100%的回收率)。此外,添加Fe(Ⅱ)T后,暴露于T5样或T4样噬菌体后存活的细菌数大于暴露于T1样或rV5样噬菌体后的细菌数(P <0.01)。因此,建议使用杀病毒剂进行噬菌体灭活,以提高对大肠杆菌O157进行生物防治的噬菌体功效评估的准确性。

著录项

  • 来源
    《Journal of food protection》 |2015年第12期|2220-2226|共7页
  • 作者单位

    China Institute of Food and Drug Control, Hohhot, Inner Mongolia, People's Republic of China, 010020,Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, Alberta, Canada T1J 4B1,Inner Mongolia Agricultural University, College of Animal Science, Hohhot, Inner Mongolia, People's Republic of China 010018;

    Inner Mongolia Agricultural University, College of Animal Science, Hohhot, Inner Mongolia, People's Republic of China 010018;

    Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, Alberta, Canada T1J 4B1,College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong, People's Republic of China 510642;

    Alberta Agriculture and Forestry, Agriculture Centre, Lethbridge, Alberta, Canada T1J 4V6;

    Inner Mongolia Agricultural University, College of Animal Science, Hohhot, Inner Mongolia, People's Republic of China 010018;

    Alberta Agriculture and Forestry, Agriculture Centre, Lethbridge, Alberta, Canada T1J 4V6;

    Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, Alberta, Canada T1J 4B1;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

  • 入库时间 2022-08-17 23:25:06

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