首页> 外文期刊>Journal of food protection >Low Temperature and Modified Atmosphere: Hurdles for Antibiotic Resistance Transfer?
【24h】

Low Temperature and Modified Atmosphere: Hurdles for Antibiotic Resistance Transfer?

机译:低温和改善的气氛:抗生素耐药性转移的障碍?

获取原文
获取原文并翻译 | 示例
       

摘要

Food is an important dissemination route for antibiotic-resistant bacteria. Factors used during food production and preservation may contribute to the transfer of antibiotic resistance genes, but research on this subject is scarce. In this study, the effect of temperature (7 to 37℃) and modified atmosphere packaging (air, 50% CO_2-50% N_2, and 100% N_2) on antibiotic resistance transfer from Lactobacillus sakei subsp. sakei to Listeria monocytogenes was evaluated. Filter mating was performed on nonselective agar plates with high-density inocula. A more realistic setup was created by performing modified atmosphere experiments on cooked ham using high-density and low-density inocula. Plasmid transfer was observed between 10 and 37℃, with plasmid transfer also observed at 7℃ during a prolonged incubation period. When high-density inocula were used, transconjugants were detected, both on agar plates and cooked ham, under the three atmospheres (air, 50% CO_2-50% N_2, and 100% N_2) at 7℃. This yielded a median transfer ratio (number of transconjugantsumber of recipients) with an order of magnitude of 10~(-4) to 10~(-6). With low-density inocula, transfer was only detected under the 100% N_2 atmosphere after 10-day incubation at 7℃, yielding a transfer ratio of 10~(-5). Under this condition, the highest bacterial density was obtained. The results indicate that low temperature and modified atmosphere packaging, two important hurdles in the food industry, do not necessarily prevent plasmid transfer from Lactobacillus sakei subsp. sakei to Listeria monocytogenes.
机译:食物是抗药性细菌的重要传播途径。食品生产和保存过程中使用的因素可能有助于抗生素抗性基因的转移,但对此问题的研究很少。在这项研究中,温度(7至37℃)和改良的气氛包装(空气,50%CO_2-50%N_2和100%N_2)对清酒乳杆菌亚种的抗生素耐药性转移具有影响。评估了单核细胞增生李斯特菌。在具有高密度接种物的非选择性琼脂平板上进行滤器交配。通过使用高密度和低密度接种物在煮熟的火腿上进行改良的气氛实验,创建了一个更现实的设置。在延长的孵育期间,在10至37℃之间观察到质粒转移,在7℃下也观察到质粒转移。当使用高密度接种物时,在7℃的三种气氛(空气,50%CO_2-50%N_2和100%N_2)下,在琼脂平板和煮熟的火腿上都检测到了转导结合物。这样得出的中值转移率(过渡结合剂数/受体数)的数量级为10〜(-4)至10〜(-6)。在低密度接种下,只有在7℃温育10天后才能在100%N_2气氛下检测到转移,转移比为10〜(-5)。在这种条件下,获得了最高的细菌密度。结果表明,低温和气调包装是食品工业中的两个重要障碍,不一定能阻止质粒从清酒乳杆菌亚种中转移。清酒单核细胞增多性李斯特菌。

著录项

  • 来源
    《Journal of food protection》 |2015年第12期|2191-2199|共9页
  • 作者单位

    Institute for Agricultural and Fisheries Research, Technology and Food Science Unit, Brusselsesteenweg 370, 9090 Melle, Belgium,Laboratory of Food Microbiology and Food Preservation, Ghent University, Coupure Links 653, 9000 Ghent, Belgium,Laboratory of Microbial Ecology and Technology, Ghent University, Coupure Links 653, 9000 Ghent, Belgium;

    Institute for Agricultural and Fisheries Research, Technology and Food Science Unit, Brusselsesteenweg 370, 9090 Melle, Belgium;

    Institute for Agricultural and Fisheries Research, Technology and Food Science Unit, Brusselsesteenweg 370, 9090 Melle, Belgium;

    Laboratory of Microbial Ecology and Technology, Ghent University, Coupure Links 653, 9000 Ghent, Belgium;

    Institute for Agricultural and Fisheries Research, Technology and Food Science Unit, Brusselsesteenweg 370, 9090 Melle, Belgium;

    Laboratory of Food Microbiology and Food Preservation, Ghent University, Coupure Links 653, 9000 Ghent, Belgium;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

  • 入库时间 2022-08-17 23:25:06

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号