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Population Dynamics of Aspergillus Section Nigri Species on Vineyard Samples of Grapes and Raisins

机译:葡萄和葡萄干葡萄园样品上黑曲霉节肢动物的种群动态

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摘要

Several species of Aspergillus section Nigri, including potential mycotoxin producers, are common residents of grape vineyards, but the relative population size of individual species throughout the growing season is difficult to determine using traditional isolation and identification methods. Using a quantitative droplet digital PCR (ddPCR) method in combination with dilution plating, total Aspergillus section Nigri populations and relative proportions of A. niger, A. welwitschiae, A. carbonarius, and A. tubingensis were measured from vineyard samples without the need for identifying individual fungal isolates. Grapes were sampled from two raisin vineyards (vineyards A and B) at berry set, veraison, harvest, and raisin stages in two consecutive years. Plate counts showed that the total population of Aspergillus section Nigri present on the fruit increased from berry set to raisin and became a larger component of the total recovered fungal population in both vineyards in both years. Results from ddPCR analysis showed that the relative proportion of A. carbonarius among the four species assayed increased later in the season (harvest and raisin) in comparison to earlier in the season (berry set and veraison). Total fungal and Aspergillus section Nigri plate counts were not significantly different between vineyards in either year. However, vineyard A generally showed higher proportions of A. carbonarius in harvest and raisin samples than vineyard B. This coincided with higher incidence and levels of ochratoxin A in vineyard A harvest and raisin fruit than in vineyard B fruit. This work demonstrates that this ddPCR method is a useful tool for culture-independent monitoring of populations of mycotoxigenic Aspergillus species during grape and raisin production.
机译:Nigri的部分黑曲霉属种,包括潜在的霉菌毒素生产者,是葡萄葡萄园的常见居民,但是使用传统的分离和鉴定方法很难确定整个生长季节中各个物种的相对种群大小。使用定量液滴数字PCR(ddPCR)方法与稀释板相结合,无需葡萄园样品就可以测量葡萄园样品中黑曲霉的总Nigri种群以及黑曲霉,细叶曲霉,碳曲霉和油曲霉的相对比例。鉴定单个真菌分离物。连续两年从两个葡萄干葡萄园(A和B葡萄园)的浆果采摘,葡萄收获期和葡萄干阶段采样葡萄。平板计数表明,在这两个葡萄园中,这两个葡萄园中,果实上存在的黑曲霉节段的总种群从浆果定植到葡萄干都增加了,并成为总回收真菌种群的较大组成部分。 ddPCR分析的结果表明,与季节较早的季节(浆果生长期和果冻季节)相比,所分析的四个物种中碳假单胞菌的相对比例在季节较晚的季节(收获和葡萄干)增加。在任何一年中,不同葡萄园的真菌和曲霉菌切片的Nigri板块总数均无显着差异。但是,葡萄园A在收获和葡萄干样品中通常显示出比葡萄B更高的A. carbonarius比例。这与葡萄园A收获和葡萄干水果中的ra曲霉毒素A发生率和水平都比葡萄园B更高。这项工作表明,这种ddPCR方法是一种有用的工具,可在葡萄和葡萄干生产过程中独立于培养物监测真菌毒素的曲霉菌种群。

著录项

  • 来源
    《Journal of food protection》 |2016年第3期|448-453|共6页
  • 作者单位

    Foodborne Toxin Detection and Prevention Research Unit, U.S. Department of Agriculture, Agricultural Research Service. 800 Buchanan Street, Albany, California 94710;

    Foodborne Toxin Detection and Prevention Research Unit, U.S. Department of Agriculture, Agricultural Research Service. 800 Buchanan Street, Albany, California 94710;

    Foodborne Toxin Detection and Prevention Research Unit, U.S. Department of Agriculture, Agricultural Research Service. 800 Buchanan Street, Albany, California 94710;

    University of California, Kearney Agricultural Research & Extension Center, 9240 South Riverbend Avenue, Parlier, California 93648, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Aspergillus carbonarius; Aspergillus niger; Droplet digital PCR; Fungal ecology; Ochratoxin;

    机译:碳曲霉;黑曲霉;液滴数字PCR;真菌生态学;ch曲毒素;
  • 入库时间 2022-08-17 23:24:46

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