MICROWAVE PRETREATMENT-ASSISTED ETHANOL EXTRACTION OF CHLOROPHYLLS FROM SPIRULINA PLATENSIS

摘要

Microwave pretreatment-assisted ethanol extraction (MPAEE) was used to extract chlorophyll a and chlorophyll b from Spirulina platensis. The effects of microwave-pretreated process and ethanol extraction process were investigated by single-factor experiments and response surface analysis experiments. The results showed that the optimum process parameters for MPAEE were: acetone/water (1:1, V/V) as the ruptured walls co-solvent (RWC), the volume of the RWC of 10 mL, microwave irradiation time of 60 s, microwave irradiation power of 560 W, extraction time of 52.8 min, extraction temperature of 55.1C, and ratio of solvent to material of 10.8 mL/g. The optimized chlorophylls extraction yield was 1.27%, matching the theoretical extraction yield. The comparison experimental results indicated that the yield of chlorophylls by MPAEE was higher than that obtained from conventional solvent extraction.