The Biodegradation of 1,3-Dichlorobenzene by an Adapted Strain Bacillus cereus PF-11 Derived from Town-Gas Industrial Effluent

摘要

In the present study, an adapted bacterium PF-11 with high 1,3-dichlorobenzene degradation capability was isolated from town-gas industrial effluent through continuous introducing of N-methyl-N′-nitro-N-nitrosoquanidine (NTG). In suitable condition, a degradation rate of 32 mg L~(-1)d~(-1) of 1,3-dichlorobenzene was obtained by strain PF-11 with effective chlorion release. Strain PF-11 was tentatively identified as gram-positive Bacillus cereus. The substrate specificity of the strain PF-11 was relatively low, and the degradation rate for different chlorobenzenes was in the order of monoehlorobenzene > 1,3-dichlorobenzene > 1,2-dichlorobenzenc. Initial oxidation step was molecular oxygen attacking chlorobcnzene ring catalyzed by dioxygenase.