首页> 外文期刊>Journal of Environmental Science and Health, Part B: Pesticides, Food Contaminants, and Agricultural Wastes >Acute toxic effects of three pesticides on Pseudomonas putida monitored by microcalorimeter
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Acute toxic effects of three pesticides on Pseudomonas putida monitored by microcalorimeter

机译:微热量计监测三种农药对恶臭假单胞菌的急性毒性作用

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A series of calorimetric experiments were performed to investigate the toxic effects of beta-cypermethrin (BCP), bensulfuron-methyl (BSM) and prometryne (PM) on Pseudomonas putida (P. putida). The metabolic action of P. putida on the three pesticides was studied by obtaining power-time curves. The growth of P. putida was inhibited completely in each case when the concentrations of pesticides were up to 80 μ g mL− 1. The relationships between the inhibitory ratio (k) and doses of contaminants were approximately linear for the three pesticides. The total heat dissipated per milliliter (Q total) for the pesticides decreased during the course of the experiment. The OD600 of P. putida growth in the absence and presence of pesticides was also obtained. The power-time curves of P. putida growth coincided with its turbidity curves. This elucidates that microcalorimetric method agrees well with the routine microbiological method. Among these three pesticides, BSM was found to be the most toxic with an IC 50 of 19.24 μ g mL− 1 against P. putida. PM exhibited moderate virulence with an IC 50 of 27.86 μ g mL− 1 and BCP had the lowest toxicity with an IC 50 of 39.64 μ g mL− 1.
机译:进行了一系列的量热实验,以研究β-氯氰菊酯(BCP),苄嘧磺隆(BSM)和炔丙基(PM)对恶臭假单胞菌(P.putida)的毒性作用。通过获得功率-时间曲线研究了恶臭假单胞菌对三种农药的代谢作用。在每种情况下,当农药的浓度高达80 µg mL â1时,恶臭假单胞菌的生长被完全抑制。三种农药的抑制率(k)和污染物剂量之间的关系近似线性。在实验过程中,每毫升农药的总散热量(Q total )降低了。还获得了在不存在和存在农药的情况下恶臭假单胞菌生长的OD 600 。恶臭假单胞菌生长的动力时间曲线与其浊度曲线一致。这说明微量量热法与常规微生物学方法非常吻合。在这三种农药中,BSM被发现对P. putida的毒性最高,IC 50 为19.24μg mL -1 。 PM表现出中等毒性,IC 50 为27.86μgmL up1 ,BCP毒性最低,IC 50 为39.64 ¼g毫升→1

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    Key Laboratory of Biogeology and Environmental Geology of Chinese Ministry of Education & School of Environmental Studies & Sino-Hungarian Joint Laboratory of Environmental Science and Health, China University of Geosciences, Wuhan, P. R. China;

    Laborato;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 入库时间 2022-08-18 00:58:05

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