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Alterations in the rabbit lymphoid tissue after bendiocarb administration

机译:施用苯达威克后兔淋巴组织的改变

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摘要

Various pesticides have immuno-suppressive effects, and thus the organisms become responsive to viral, bacterial and parasitic diseases and neoplasm. The aim of the study was to observe the structure of the small intestine (height of enterocytes and crypts), mucosal lymphoid tissue (Payer's patches, lymphocytes in lamina propria) and a lymph node after administration of bendiocarb (2,2-dimethyl-1,3-benzodioxol-4-yl-methylcarbamate) on days 3, 10, 20, 30 and 60 of the experiment. The height of the observed enterocytes showed an increasing tendency. On days 20, 30 and 60 we also observed an increase in diameter of crypts located in intestinal epithelium. The number of cells in lamina propria mucosae was significantly reduced on days 20 and 30 after administration of bendiocarb. Observations of the lymph node showed that on days 10 and 20 there was a significant increase in relative volume of medulla at the expense of the relative volume of the cortex and a decrease in the number of lymphocytes. However, we recorded an increase in diameter of lymphocytes. The intracellular parasite Toxoplasma gondii (T. gondii) belongs to the most common pathogenic parasites in the world and it can cause serious health complications in pregnant and immunodeficient individuals. DNA isolation, standard polymerase chain reaction (PCR) and visualization in a 2.5 % agarose gel, the presence of DNA T. gondii was detected in no examined rabbit brain samples. Using real time PCR T. gondii DNA was detected and quantified in the three rabbit brain samples (10 %).
机译:各种农药具有免疫抑制作用,因此生物体对病毒,细菌和寄生虫病以及肿瘤有反应。这项研究的目的是观察服用苯达威(2,2-二甲基-1)后小肠的结构(肠细胞和隐窝的高度),粘膜淋巴组织(Payer's patch,固有层的淋巴细胞)和淋巴结的结构在实验的第3、10、20、30和60天加入(3-3-苯并二恶唑-4-基-甲基氨基甲酸酯)。观察到的肠上皮细胞的高度呈增加趋势。在第20、30和60天,我们还观察到位于肠上皮的隐窝直径增大。施用苯达威克后第20天和第30天,固有黏膜中的细胞数量明显减少。淋巴结的观察表明,在第10天和第20天,髓质的相对体积显着增加,但皮质的相对体积却减少了,而淋巴细胞的数量却减少了。但是,我们记录了淋巴细胞直径的增加。细胞内寄生虫弓形虫(T. gondii)属于世界上最常见的致病性寄生虫,它可以在怀孕和免疫缺陷的个体中引起严重的健康并发症。在2.5%的琼脂糖凝胶中进行DNA分离,标准聚合酶链反应(PCR)和可视化,在未检查的兔脑样本中检测到DNA弓形虫的存在。使用实时PCR检测弓形虫DNA,并在三个兔脑样本(10%)中进行定量。

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    Institute of Anatomy, University of Veterinary Medicine and Pharmacy, Kosice, Slovak Republic;

    Institute of Biology, University of Veterinary Medicine and Pharmacy, Kosice, Slovak Republic;

    Institute of Histology and Embryology, University of Veterinary;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 入库时间 2022-08-18 00:58:00

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