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Development of an efficient extraction method for oxytetracycline in animal manure for high performance liquid chromatography analysis

机译:高效液相色谱分析动物粪便中土霉素的有效提取方法的建立

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摘要

Oxytetracycline (2-(amino-hydroxy-methylidene)-4-dimethylamino-5,6,10,11,12a-pentahydroxy-6-methyl-4,4a,5,5a-tetrahydrotetracene-1,3,12-trione) is a major member of the tetracycline antibiotics family of which are widely administered to animals in concentrated animal feeding operations for purposes of therapeutical treatment and health protection. With the disposal of animal manure as fertilizer into agricultural land, tetracyclines enter the environment. However, tetracyclines chelate with multivalent cations and proteins, resulting in low extraction efficiencies from animal manure for tetracycline residue analysis. In this study an efficient extraction method for oxytetracycline from steer manure using methanol/water solution amended with chelating organic acid was developed for the analysis of high performance liquid chromatography. The effect of species and amount of amendment acids, shaking time, methanol/water ratio, manure weight, and repeated times of extraction was investigated. It was optimized to amend 2.5 g citric acid and 1.1 g oxalic acid with 10.0 g manure sample in a 50-ml centrifuge tube and extract with 15 ml methanol/water (9:1 in volume) by vigorously shaking for 30 min in a reciprocating shaker. After centrifugation at 11,000 rpm, supernatant is collected. Sample was extracted for a total of 3 times. The developed extraction method was further applied to extract oxytetracycline from fresh and aged cow manure, swine and poultry manure, and soil. Satisfactory recoveries ranging from (84.1 ± 2.4) % to (102.0 ± 3.1) % were obtained, demonstrating that the optimized extraction method is robust for oxytetracycline from different manure sample matrixes.
机译:土霉素(2-(氨基-羟基-亚甲基)-4-二甲基氨基-5,6,10,11,12a-五羟基-6-甲基-4,4a,5,5a-四氢并四苯-1,3,12-三酮)是四环素抗生素家族的主要成员,其在浓缩动物饲养操作中广泛应用于动物,以进行治疗和保护健康。随着将动物粪便作为肥料处置进入农田,四环素进入了环境。然而,四环素与多价阳离子和蛋白质螯合,导致动物粪便中四环素残留分析的提取效率低。在这项研究中,开发了一种高效的从牛粪中提取土霉素的方法,该方法是使用甲醇/含螯合有机酸的水溶液修饰的高效液相色谱分析方法。研究了改性酸的种类和数量,振摇时间,甲醇/水比,粪肥重量和重复提取次数的影响。它经过优化,可在50 ml离心管中与10.0 g粪便样品一起修正2.5 g柠檬酸和1.1 g草酸,并通过在往复运动中剧烈摇动30分钟而用15 ml甲醇/水(体积比为9:1)提取摇床。以11,000 rpm离心后,收集上清液。样品共提取3次。进一步开发的提取方法进一步应用于从新鲜和老化的牛粪肥,猪和家禽粪便以及土壤中提取土霉素。令人满意的回收率范围从(84.1±2.4)%到(102.0±3.1)%,表明优化的提取方法对于从不同粪便样品基质中的土霉素而言是稳健的。

著录项

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  • 作者

    Shoujun Yuan;

  • 作者单位

    Chemistry Department, Delaware State University, Dover, Delaware, USA;

    School of Civil Engineering, Hefei University of Technology, Anhui, China;

    US Department of Agriculture, Agricultural Research Service Salinity Laboratory, Riverside, California, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

  • 入库时间 2022-08-18 00:57:59

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