首页> 外文期刊>Journal of Environmental Science and Health, Part B: Pesticides, Food Contaminants, and Agricultural Wastes >Characterization of microbial consortia that reductively dechlorinate 4-chlorophenol and transform phenol to benzoate enriched from estuarine sediment of Lake Shinji
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Characterization of microbial consortia that reductively dechlorinate 4-chlorophenol and transform phenol to benzoate enriched from estuarine sediment of Lake Shinji

机译:微生物财团的表征,该财团可还原新氯湖河口沉积物中富集的4-氯苯酚并将氯转化为苯甲酸酯

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摘要

Monochlorophenols were degraded to benzoate via phenol by the initial dechlorination and the subsequent conversion of phenol to benzoate in anaerobic sediment samples of estuarine Lake Shinji under methanogenic conditions. To characterize bacteria that dechlorinate 4-chlorophenol and transform phenol to benzoate, we analyzed the microbial community structure of the enrichment culture with each 4-chlorophenol and phenol by the limiting dilution method with polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) of 16S rRNA gene. After serial dilution of the culture, the 4-chlorophenol-dechlorinating culture consisted of two dominant bacteria, one of which was most homologous with Dehalobacter sp. In the enriched culture with phenol, minor band homologous with Cryptanaerobacter phenolicass corresponded to the transformation activity.
机译:在产甲烷条件下,河口Shinji湖厌氧沉积物样品中的初始脱氯和随后苯酚转化为苯甲酸酯的过程中,一氯酚经苯酚降解为苯甲酸酯。为了表征对4-氯苯酚进行脱氯并将苯酚转化为苯甲酸酯的细菌,我们采用聚合酶链反应-变性梯度凝胶电泳(PCR-DGGE)有限稀释法分析了每种4-氯苯酚和苯酚的富集培养物中的微生物群落结构。 16S rRNA基因的克隆。在连续稀释培养物后,4-氯苯酚脱氯培养物由两种优势细菌组成,其中一种与Dehalobacter sp。最为同源。在富含酚的培养物中,与酚醛隐孢菌同源的次要带对应于转化活性。

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