Our Industry Today: Fertility of Bull Sperm Frozen and Stored in Clarified Egg Yolk-Tris-Glycerol Extender

摘要

Egg yolk-Tris-glycerol extender, which is widely used in commercial artificial insemination (AI), was modified by replacing the 20/100 egg yolk (vol/vol) with a supernatant from egg yolk centrifuged at 50,000 × g for 2 h and then used to cryopreserve bull sperm. Preliminary studies showed that 20/100 egg yolk inter- Fered with biochemical assays, which could be over- Come by centrifugation. In Experiment 1, semen from 4 Holstein bulls was frozen in the experimental Tris extender and compared with the whole milk-glycerol control.