Crystallzation of high molecular weight urokinase

摘要

Urokinase-type plasminogen activator (u-PA), urokinase, belongs to difficult crystallizing proteins. Many efforts were made in the last decade to grow high molecular weight (molecular weight, 54 kDa) u-PA crystals of appropriate quality and sizes. But usual results were small crystals with sizes abotu 0.05 mm grown very slowly, during several months. This paper presents big u-PA crystals, of sizes ￣ 0.3-0.8 mm grown in form several day to 1.5 week. The crystal were grown insideglass capillaries and have rectangular parallelepiped or, in may cases, cubic external shape.