Optimization of Preparative Separation and Purification of Total Flavonoids from Radix Puerariae by Macroporous Resin Method

摘要

Aim To screen the optimum macroporous resin and conditions for the isolation and purification of flavonoids from Radix Puerariae. Methods The static and dynamic adsorption/desorp-tion methods were used, and the separation and purification process was evaluated by measuring the concentration of total flavonoid in the fractions with UV spectrophotometer. Results The SP70 macro-porous resin was the most effective compared with other macroporous resins. The optimum conditions were screened, which were 0.5 g · mL~(-1) corresponding to crude drug for concentration of extract, pH 5 - 6, and appended 60 times the volume of the resin bed ( BV ) with the adsorption speed 2 BV · h~(-1), and the volume of aq. 70% ( V/V) ethanol as eluant was 5 BV with desorption speed 2 BV·h~(-1). By this method, the final contents of total flavonoids exceeded 80%. Conclusion The SP70 macroporous resin is the most effective one for large-scale isolation and purification of flavonoids from Radix Puerar-ia, which meets industrial needs.