Heme-regulated eukaryotic initiation factor 2α kinase-a molecular indicator of haemolytic anemia

摘要

The heme-regulated eukaryotic initiation factor-2α (eIF-2α) kinase, also called the heme-regulated inhibitor (HRI), is a key regulator of protein synthesis in mammalian reticulocyte. HRI is almost undetectable in blood samples of normal rabbits and it increases by 12 15-fold in the reticulocytes of anemic rabbits. In order to determine if such an increase in the quantity of HRI is gradual during anemia, and if it could be an indicator of anemia, we have carried out a detailed analysis on the expression of HRI and its eIF-2α kinase activity in rabbit reticulocyte lysates during various stages of acetylphenylhydrazine (APH)-indnced anemia.