Immunoprophylactic studies on cell wall associated proteins of Mycobacterium tuberculosis H_37Ra

摘要

The cell wall protein peptidoglycan complex (CW-PPC) of Mycobacterium tuberculosis H_37Ra was isolated through sequential extraction of lipids, carbohydrates and soluble proteins. CW-PPC emulsified in FIA was found to induce significant protection in mice against challenge with LD_50 dose of M. tuberculosis H_37Rv. To identify the immunoprotective components of CW-PPC, the proteins in avid association with peptidoglcan were dissociated by chemical treatment with trifluoromethanesulthonic acid (CF_3CO_3H): anisole (2:1). Immunoreactivity of total (CW-Pr) as well as its component proteins i.e., 71, 60 and 45kDa proteins of cell wall was studied in animals immunized with CW-Pr-FIA.