首页> 外文期刊>Journal of Biosciences >Transgenic tobacco plants expressing BoRS1 gene from Brassica oleracea var. acephala show enhanced tolerance to water stress
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Transgenic tobacco plants expressing BoRS1 gene from Brassica oleracea var. acephala show enhanced tolerance to water stress

机译:表达来自甘蓝型油菜的BoRS1基因的转基因烟草植物。头足动物对水分胁迫的耐受性增强

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摘要

Water stress is by far the leading environmental stress limiting crop yields worldwide. Genetic engineering techniques hold great promise for developing crop cultivars with high tolerance to water stress. In this study, the Brassica oleracea var. acephala BoRS1 gene was transferred into tobacco through Agrobacterium-mediated leaf disc transformation. The transgenic status and transgene expression of the transgenic plants was confirmed by polymerase chain reaction (PCR) analysis, Southern hybridization and semi-quantitative one step RT-PCR analysis respectively. Subsequently, the growth status under water stress, and physiological responses to water stress of transgenic tobacco were studied. The results showed that the transgenic plants exhibited better growth status under water stress condition compared to the untransformed control plants. In physiological assessment of water tolerance, transgenic plants showed more dry matter accumulation and maintained significantly higher levels of leaf chlorophyll content along with increasing levels of water stress than the untransformed control plants. This study shows that BoRS1 is a candidate gene in the engineering of crops for enhanced water stress tolerance.
机译:迄今为止,水分胁迫是限制全球作物产量的主要环境胁迫。基因工程技术对发展对水分胁迫具有高度耐受性的作物品种具有广阔的前景。在这项研究中,甘蓝变种。通过农杆菌介导的叶盘转化将无头型BoRS1基因转移到烟草中。通过聚合酶链反应(PCR)分析,Southern杂交和半定量RT-PCR分析分别证实了转基因植物的转基因状态和转基因表达。随后,研究了水分胁迫下转基因烟草的生长状况以及对水分胁迫的生理响应。结果表明,与未转化的对照植物相比,转基因植物在水分胁迫条件下表现出更好的生长状态。在对水分耐受性的生理评估中,与未转化的对照植物相比,转基因植物显示出更多的干物质积累,并保持着明显更高的叶片叶绿素含量以及水分胁迫水平的提高。这项研究表明,BoRS1是增强水分胁迫耐受性的作物工程候选基因。

著录项

  • 来源
    《Journal of Biosciences》 |2005年第5期|p.647-655|共9页
  • 作者单位

    School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 201101, People's Republic of China.;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物科学;
  • 关键词

  • 入库时间 2022-08-17 23:37:31

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