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Assessing photosynthesis by fluorescence imaging

机译:通过荧光成像评估光合作用

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This practical paper describes a novel fluorescence imaging experiment to study the three processes of photochemistry, fluorescence and thermal energy dissipation, which compete during the dissipation of excitation energy in photosynthesis. The technique represents a non-invasive tool for revealing and understanding the spatial heterogeneity in leaf performance caused by internal and external factors, such as development and abiotic stress. Fluorescence yield (F), effective PSII quantum yield (Y(II)) and non-photochemical quenching (NPQ) were analysed by fluorescence imaging in leaves of Avena sativa L. and two species of Brassica (B. fruticulosa and B. oleracea) plants during the first stages of plant development and under high illumination. In young leaves, F was 0.1 or less, Y(II) between 0.7 and 0.8 and NPQ less than 0.3, indicating that photochemical efficiency was high (70-80%) and that fluorescence emission (less than 10%) and heat dissipation (less than 30%) were low. As leaf age increased, differences were observed between species, indicating different development and senescence patterns in each. Avena sativa and B. fruticulosa (a wild species adapted to high light intensity in its natural habitat) showed greater tolerance to high illumination than B. oleracea (an agricultural species), which experienced photoinhibition caused by excessive light. This practical paper is intended for advanced students of plant biochemistry and plant physiology.View full textDownload full textKeywordsAvena sativa, Brassica , high light intensity, photosystemRelated var addthis_config = { ui_cobrand: "Taylor & Francis Online", services_compact: "citeulike,netvibes,twitter,technorati,delicious,linkedin,facebook,stumbleupon,digg,google,more", pubid: "ra-4dff56cd6bb1830b" }; Add to shortlist Link Permalink http://dx.doi.org/10.1080/00219266.2010.546009
机译:该实用论文描述了一种新颖的荧光成像实验,以研究光化学,荧光和热能耗散的三个过程,它们在光合作用的激发能耗散过程中相互竞争。该技术代表了一种非侵入性工具,用于揭示和理解由内部和外部因素(例如发育和非生物胁迫)引起的叶片性能的空间异质性。通过荧光成像分析了燕麦和两种芸苔属植物(金黄色葡萄球菌和油橄榄)的荧光产量(F),有效PSII量子产量(Y(II))和非光化学猝灭(NPQ)。植物在植物发育的第一阶段并处于高光照下。在幼叶中,F等于或小于0.1,Y(II)在0.7和0.8之间,NPQ小于0.3,表明光化学效率高(70-80%),并且荧光发射(小于10%)和散热(低于30%)的人。随着叶龄的增加,在物种之间观察到差异,表明每种物种的发育和衰老模式不同。相较于油菜B. oleracea(一种农业物种),Avena sativa和B. fruticulosa(一种在其自然栖息地适应高光强度的野生物种)对高光照的耐受性更高,后者由于过量的光而受到光抑制。本实用程序文章适用于植物生物化学和植物生理学的高级学生。查看全文下载全文关键字Avena sativa,Brassica,high light strength,photosystemRelated var addthis_config = {ui_cobrand:“ Taylor&Francis Online”,services_compact:“ citeulike,netvibes,twitter ,technorati,可口,linkedin,facebook,stumbleupon,digg,google,更多”,发布号:“ ra-4dff56cd6bb1830b”};添加到候选列表链接永久链接http://dx.doi.org/10.1080/00219266.2010.546009

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