Identification and Differentiation of Hop Varieties Using Simple Sequence Repeat Markers

摘要

Microsatellites are used in the current study as molecular markers for identifying hop varieties. Sixty-three hop accessions were analyzed by using four primer pairs for the microsatellite analysis, and an additional 10 primer pairs were used for the analysis of 16 subgroup accessions. Altogether, 73 alleles were amplified, of which 14 were specific for nine cultivars. Overall, 116 genotypes were observed, of which 50 were unique and detected only once. For cultivar identification, a minimum of five primer pairs was selected, which allowed the discrimination of 44 cultivars. The representatives of clonal or mass selection (Sazzer's and Fuggle's group of cultivars, Goldings, and Japanese and Chinese varieties) revealed the same banding patterns; similarly, all tetraploids gave the same pattern as their diploid donor. The discrimination power of microsatellites is shown on an example of planting material, in which phenotypic variation was confirmed on a genetic level. The main purpose of this research is to provide a scheme of cultivar identification based on polymorphic microsatellite data useful for hop breeders, merchants, and brewers.