A Rapid and Low-Cost Method for Quantification of Reduced Iso-α-Acids in Brewing

摘要

A rapid HPLC method for routine wort and beer analysis during production has been developed. The method is based on the separation of the reduced iso-α-acids such as dihydro-, tetrahydro-, and hexahydro-iso-α-acids. This is done by means of a new column technology and direct injection of the sample. The new method is significantly faster and more economical than are the existing methods, obtaining 86% savings in time and 60% savings in cost. The method has been validated and implemented in our five laboratories with excellent results in repeatability and reproducibility.