An Efficient Methodology for the Preparation of Alkoxyglycerols Rich in Conjugated Linoleic Acid and Eicosapentaenoic Acid

摘要

Saponification of a raffinate obtained by supercritical fluid fractionation of shark liver oil was utilized for the production of an unsaponifiable fraction enriched in nonesterified alkoxyglycerols. Re-esterification of the alkoxyglycerols produced with conjugated linoleic acid (CLA) and eicosapentaenoic acid in the presence of Candida antarctica lipase B was then studied. High yields (>90%) of diesterified alkoxyglycerols were obtained via esterification and transesterification with CLA, their respective ethyl ester, and eicosapentaenoic ethyl ester. The effect of a continuous purge stream of nitrogen on the kinetics of the reactions was also evaluated.