Deconvolution of Combinatorial Oligonucleotide Libraries by Electrospray Ionization Tandem Mass Spectrometry

摘要

Studies were undertaken to explore the application of tandem mass spectrometry for the structure analysis of unfractionated mixtures of oligonucleotides. Limited combinatorial libraries were constructed of mixtures of 8-mers (NGACACNG; nine compounds) and 12-mers (NGACTNAGACNG; 27 compounds), where N is any of the 2'-deoxyribonucleotides of uracil, thymine, or 5-[N-(aminoethyl)-3-acrylimido]uracil.