Supramolecular Interactions Probed by 13C−13C Solid-State NMR Spectroscopy

摘要

We present a robust solid-state NMR approach for the accurate determination of molecular interfaces in insoluble and noncrystalline protein−protein complexes. The method relies on the measurement of intermolecular 13C−13C distances in mixtures of [1-13C]glucose- and [2-13C]glucose-labeled proteins. We have applied this method to Parkinson’s disease-associated α-synuclein fibrils and found that they are stacked in a parallel in-register arrangement. Additionally, intermolecular distance restraints for the structure determination of the fibrils at atomic resolution were measured.