Fluorinated Diaminocyclopentanes as Chiral Sensitive NMR Probes of RNA Structure

摘要

The supramolecular chiral recognition between rac-2a and several structured RNA leads to a distinct 19F NMR signal splitting. The 19F NMR analysis of the diastereomeric pairs formed upon binding of this racemic probe delivers a topological footprint of the RNA. This phenomenon can be exploited to investigate dynamic events involving structural equilibria, as demonstrated in a melting experiment. This work provides a proof of concept that small fluorinated moderate binders can act as external probes of RNA structures.