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Introducing a 2-His-1-Glu Nonheme Iron Center into Myoglobin Confers Nitric Oxide Reductase Activity

机译:将2-His-1-Glu非血红素铁中心引入肌红蛋白赋予一氧化氮还原酶活性

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摘要

A conserved 2-His-1-Glu metal center, as found in natural nonheme iron-containing enzymes, was engineered into sperm whale myoglobin by replacing Leu29 and Phe43 with Glu and His, respectively (swMb L29E, F43H, H64, called FeBMb(-His)). A high resolution (1.65 Å) crystal structure of Cu(II)-CN−-FeBMb(-His) was determined, demonstrating that the unique 2-His-1-Glu metal center was successfully created within swMb. The FeBMb(-His) can bind Cu, Fe, or Zn ions, with both Cu(I)-FeBMb(-His) and Fe(II)-FeBMb(-His) exhibiting nitric oxide reductase (NOR) activities. Cu dependent NOR activity was significantly higher than that of Fe in the same metal binding site. EPR studies showed that the reduction of NO to N2O catalyzed by these two enzymes resulted in different intermediates; a five-coordinate heme-NO species was observed for Cu(I)-FeBMb(-His) due to the cleavage of the proximal heme Fe-His bond, while Fe(II)-FeBMb(-His) remained six-coordinate. Therefore, both the metal ligand, Glu29, and the metal itself, Cu or Fe, play crucial roles in NOR activity. This study presents a novel protein model of NOR and provides insights into a newly discovered member of the NOR family, gNOR.
机译:在天然的含非血红素铁的酶中发现一个保守的2-His-1-Glu金属中心,通过分别用Glu和His取代Leu29和Phe43(swMb L29E,F43H,H64,称为Fe < sub> B Mb(-His))。确定了Cu(II)-CN - -Fe B Mb(-His)的高分辨率(1.65Å)晶体结构,表明独特的2-His-在swMb中成功创建了1-Glu金属中心。 Fe B Mb(-His)可以结合Cu,Fe或Zn离子,同时与Cu(I)-Fe B Mb(-His)和Fe(II) )-Fe B Mb(-His)表现出一氧化氮还原酶(NOR)活性。在相同的金属结合位点,Cu依赖性NOR活性显着高于Fe。 EPR研究表明,这两种酶将NO还原为N 2 O导致了不同的中间体。 Cu(I)-Fe B Mb(-His)的五配位血红素-NO物种由于近端血红素Fe-His键的断裂而被观察到,而Fe(II)-Fe B Mb(-His)保持六坐标。因此,金属配体Glu29和金属本身Cu或Fe在NOR活性中都起着至关重要的作用。这项研究提出了一种新型的NOR蛋白质模型,并提供了对NOR家族的新成员gNOR的见识。

著录项

  • 来源
    《Journal of the American Chemical Society》 |2010年第29期|p.9970-9972|共3页
  • 作者

    Ying-Wu Linrs;

  • 作者单位

    Department of Chemistry, George L. Clark X-ray Facility & 3M Materials Lab, Department of Biochemistry, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, and Department of Biology, Brookhaven National Laboratory, Upton, New York 11973;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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  • 入库时间 2022-08-18 00:50:19

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