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Mechanical Distortion of Protein Receptor Decreases the Lifetime of a Receptor−Ligand Bond

机译:蛋白质受体的机械变形减少了受体-配体键的寿命

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摘要

Substantial experimental evidence indicates that the mechanical force applied to pull apart non-covalent molecular bonds (such as receptor−ligand pairs) can significantly decrease the bond lifetime. This evidence is often generated in single-molecule experiments that are designed to specifically test effects of pulling forces. However, the effect of compressive forces on the lifetime of receptor−ligand bonds remains largely unexplored. Here we extend the common usage of the atomic force microscopy technique to study whether compressive forces applied to bound streptavidin−biotin species can significantly accelerate the rate of dissociation. Presented experimental data indicate that compressive forces can substantially decrease the lifetime of the molecular bond. Surprisingly, the efficiency of accelerating dissociation by compressive forces sometimes exceeds the enhancement of the dissociation rate measured in pulling experiments, indicating that compressive forces applied to the bound species might be efficiently used to control the lifetime of adhesion bonds.
机译:大量的实验证据表明,拉开非共价分子键(例如受体-配体对)的机械力会大大缩短键的寿命。该证据通常是在单分子实验中产生的,这些实验旨在专门测试拉力的影响。然而,压缩力对受体-配体键寿命的影响在很大程度上尚待探索。在这里,我们扩展了原子力显微镜技术的通用用法,以研究施加于结合的链霉亲和素-生物素种类的压缩力是否可以显着加快解离速率。提出的实验数据表明,压缩力会大大降低分子键的寿命。出人意料的是,通过压缩力加速离解的效率有时超过了在拉力实验中测得的离解速率的提高,这表明施加到结合物种上的压缩力可有效地用于控制粘合寿命。

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