Detection of furfural and 5-hydroxymethylfurfural with a yhcN::luxCDABE bioreporter strain

摘要

RT-qPCR data demonstrated that the yhcN gene was highly expressed when Escherichia coli was exposed to 500 ppm of ferulic acid (9.8-fold), vanillin (7.3-fold) or furfural (2.2-fold). Consequently, an E. coli yhcN::lux bioreporter strain (DMY1) was constructed. This strain is very responsive to furfural and 5-hydroxymethylfurfural, showing a maximum induced bioluminescence of 29.4- and 17.8-fold, respectively. The responses to different phenolics were also significant, with relative responses of between 4- and 16-fold. In tests with rice straw hydrolysate samples, a dual induction of DMY1 was observed and it was demonstrated that this corresponded to the activities of the phenolics and HMF within the sample. The maximum response seen with the hydrolysate sample was 3.5 and the minimum phenolic concentration detected was 39 mg/L. These results illustrate that this strain can be used to monitor for the presence of furans and phenolics commonly found within plant hydrolysate samples.