FUNDAMENTAL STUDIES OF DIELECTRIC CHARACTERISTICS OF HEAT-INJURED S. CEREVISIAE USING DIELECTROPHORETIC IMPEDANCE MEASUREMENT METHOD

摘要

Rapid and high sensitivity methods for microorganism detection and constant monitoring system in fermentation processes have been required for advanced quality preservation in food and beverage industries. In the present work, the metabolic states of heat-injured Saccharomyces cerevisiae (5. cerevisiae) in a micro-cell were investigated using dielectrophoretic impedance measurement (DEPIM) method. Temporal change in the conductance between micro-gap (AG) was measured for various heat treatment temperatures (HTT). As a result, there is an obvious correlation between HTT and AG as well as our previous works for Escherichia coli (E. coli). These results suggest that DEPIM method should be available for an effective monitoring method for complex change in various biological states of microorganisms.