A simple and sensitive method for the spectrophotometric determination of diosmin has been developed, and optimum reaction conditions along with other analytical parameters have been evaluated. Diosmin, as a phenolic compound, reacts with sodium nitroprusside and hydroxylamine hydrochloride in a basic medium to give a green-colored product. At analytical wavelength of 763 nm, Beer's law is obeyed over the concentration range 2-140 μg mLâ1 of diosmin with a correlation coefficient of 0.9997. The molar absorptivity is 9671 L molâ1 cmâ1. The limits of detection (LOD) and quantification (LOQ) are 0.05 and 0.2. μg mLâ1, respectively. The method has been successfully applied to the determination of diosmin in a commercial pharmaceutical preparation. The results were compared favorably with those obtained by the 4-aminoantipyrine method.View full textDownload full textKeywordsDiosmin, hydroxylamine hydrochloride, sodium nitroprusside, spectrophotometryRelated var addthis_config = { ui_cobrand: "Taylor & Francis Online", services_compact: "citeulike,netvibes,twitter,technorati,delicious,linkedin,facebook,stumbleupon,digg,google,more", pubid: "ra-4dff56cd6bb1830b" }; Add to shortlist Link Permalink http://dx.doi.org/10.1080/10739149.2010.545851
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机译：开发了一种简单灵敏的分光光度法测定薯di总皂甙的方法，并已对最佳反应条件以及其他分析参数进行了评估。作为一种酚类化合物，薯os皂素与硝普钠和盐酸羟胺在碱性介质中反应，得到绿色产物。在763 nm的分析波长处，比奥斯定的浓度范围为2-140μgmL â1’1 的薯精的比尔定律，相关系数为0.9997。摩尔吸收率是9671 L mol -1> scm -1> sup>。检出限（LOD）和定量（LOQ）分别为0.05和0.2.ug g mL →1 。该方法已成功应用于商业药物制剂中薯di的测定。结果与用4-氨基安替比林方法获得的结果相吻合。 technorati，delicious，linkedin，facebook，stumbleupon，digg，google，更多”，发布：“ ra-4dff56cd6bb1830b”};添加到候选列表链接永久链接http://dx.doi.org/10.1080/10739149.2010.545851
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