M?ssbauer spectroscopy with high velocity resolution in the study of ferritin and Imferon: preliminary results

摘要

An increase of velocity resolution in M?ssbauer spectroscopy allows increasing accuracy and decreasing experimental error in determination of hyperfine parameters. Lyophilized samples of human liver ferritin and Imferon (Fisons UK) were measured at room temperature by M?ssbauer spectroscopy with high velocity resolution. Spectra were measured in 4,096 channels for further presentation in 256, 512, 1,024, 2,048 and 4,096 channels. The differences of quadrupole splitting and isomer shift revealed for both ferritin and Imferon M?ssbauer spectra presented in 512 and 2,048 channels, respectively, were much better distinguished in case of higher velocity resolution. Therefore, M?ssbauer spectroscopy with high velocity resolution may be very useful to reveal small differences of hyperfine parameters for biological subjects in biomedical research.