Effect of Opioid Peptides on Oxygen-Dependent Microbicidal Activity of Peripheral Blood Neutrophils

摘要

The effect of opioid peptides on the production of reactive oxygen by neutrophils in a nonfrac-tionated leukocyte suspension and in a purified fraction of peripheral blood neutrophils was studied. Selective 8- and μ-agonists of peptide origin were shown to stimulate the spontaneous and inhibit zymosan-induced (15 μg/Ml) LDCL (luminol-dependent chemiluminescence) reactions of neutrophils in leukocyte suspension. (β-Endorphin caused a weaker inhibitory effect on zymosan-induced (15 μg/Ml) LDCL, and the δ_2-agonost deltorphin II stimulated zymosan-induced (15 μg/Ml) LDCL only for 25 min of the experiment. Β-Endorphin and selective δ- and μ-agonists did not affect spontaneous or inhibited zymosan-induced (15 and 150 μg/Ml) LDCL in a purified fraction of peripheral blood neutrophils. Therefore, opioid peptides play an essential role in direct and indirect regulation of the oxygen-dependent system of neutrophil granu-locyte bactericidal activity.