Evaluation of Angiotensinogen c.1-44G>A and p.M268T Variants as Risk Factors for Fibrosis Progression in Chronic Hepatitis C and Liver Diseases of Various Etiologies

摘要

Background: Hepatic stellate cells express all components of the renin-angiotensinogen (AGT) system and secrete active angiotensin II. Animal studies provided evidence that angiotensin II stimulates the accumulation of extracellular matrix by enhancing transforming growth factor β1 production. A functional genetic alteration in the human AGT promoter (c.l-44G>A) has been linked to accelerated progression of fibrosis in hepatitis C virus infection. Methods: We enrolled 2154 patients with chronic liver disease of various etiologies, including 1286 individuals with chronic hepatitis C virus infection as well as 207 healthy volunteers. We performed genotyping for two AGT variants, c.l-44G>A and c.803T>C (p.M268T), by melting curve analysis using fluorescence resonance energy transfer probes. Results: Allele frequencies and genotype distributions of both variants did not differ between patients and controls. Genotype frequencies of the c.l-44G>A variant were GG 31.0%, GA 45.6%, and AA 23.4% in patients and GG 30.0%, GA 47.8%, and AA 22.2% in controls. The genotype frequencies of p.M268T, which is in strong linkage disequilibrium with c.l-44G>A, were MM 30.8%, MT 45.5%, and TT 23.4% in patients and MM 29.0%, MT 48.8%, and TT 22.2% in controls. Both variants were associated with neither higher stages of fibrosis nor requirement for liver transplantation in any of the diagnosis subgroups. Particularly, these genetic alterations were not associated with progressive fibrosis in chronic HCV infection. Conclusion: In contrast to previous reports, both AGT variants do not predispose to the progression of fibrosis in chronic liver disease.