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Association of Genetic Polymorphisms in Matrix Metalloproteinase-9 and Coronary Artery Disease in the Chinese Han Population: A Case-Control Study

机译:中国汉族人群基质金属蛋白酶9基因多态性与冠状动脉疾病的关联

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摘要

Objective: Matrix metalloproteinase-9 (MMP-9) plays an important role in inflammation and matrix degradation involved in atherosclerosis and plaque rupture. The T allele of rs3918242 has been reported to lead to a high promoter activity and associate with the extent of coronary artery disease (CAD). And some studies have reported that the G allele of rsl7576 might be associated with CAD. The aim of this study was to assess the association between the polymorphisms of the MMP-9 gene and CAD in the Chinese Han population. Methods: This case-control study comprised 258 CAD cases and 153 controls from the Chinese Han Population. The genomic DNA of MMP-9 was isolated from whole blood. Polymerase chain reaction-based restriction fragment length polymorphism was used to determine the rs3918242 and rsl7576 genotypes in the MMP-9 gene and the total serum levels of MMP-9 were measured using enzyme-linked immunosorbent assay in both case and control groups. Results: Analysis of MMP-9 gene polymorphisms showed that the frequencies of the T allele and CT+TT genotypes of rs3918242 were significantly higher in the case group than in the control group (p < 0.05). However, the distribution of variant genotypes of rsl7576 did not differ between the case and control groups (p > 0.05). The total serum level of MMP-9 was significantly higher in the case group than in the control group (p<0.05). The subjects carrying T alleles in the CAD group had higher average serum MMP-9 levels compared with CC genotypes (p<0.05). Conclusions: Our results suggest that the single-nucleotide polymorphism of rs3918242 in the MMP-9 gene is associated with CAD and high serum levels of MMP-9 are also associated with CAD in the Chinese Han population. Therefore, genetic variation of rs3918242 may participate in the development of CAD through influencing MMP-9 expression.
机译:目的:基质金属蛋白酶9(MMP-9)在涉及动脉粥样硬化和斑块破裂的炎症和基质降解中起重要作用。 rs3918242的T等位基因据报道可导致高启动子活性,并与冠状动脉疾病(CAD)程度有关。并且一些研究报道了rsl7576的G等位基因可能与CAD有关。这项研究的目的是评估中国汉族人群MMP-9基因多态性与CAD之间的关联。方法:本病例对照研究包括258例CAD病例和153例中国汉族人群的对照。从全血中分离出MMP-9的基因组DNA。基于聚合酶链反应的限制性片段长度多态性用于确定MMP-9基因中的rs3918242和rsl7576基因型,并在病例组和对照组中使用酶联免疫吸附法测定了MMP-9的总血清水平。结果:MMP-9基因多态性分析显示,病例组rs3918242的T等位基因频率和CT + TT基因型频率明显高于对照组(p <0.05)。但是,rsl7576的变异基因型分布在病例组和对照组之间没有差异(p> 0.05)。病例组的血清总MMP-9水平明显高于对照组(p <0.05)。与CC基因型相比,CAD组中携带T等位基因的受试者的平均血清MMP-9水平更高(p <0.05)。结论:我们的结果表明,中国汉族人群MMP-9基因rs3918242的单核苷酸多态性与CAD相关,MMP-9高血清水平也与CAD相关。因此,rs3918242的遗传变异可能通过影响MMP-9表达而参与CAD的发展。

著录项

  • 来源
    《Genetic testing and molecular biomarkers》 |2013年第9期|707-712|共6页
  • 作者单位

    Department of Cardiology, First Hospital, Jilin University, Changchun, China;

    Norman Bethune College of Medicine, Jilin University, Changchun, China;

    Department of Cardiology, First Hospital, Jilin University, Changchun, China;

    Department of Cardiology, First Hospital, Jilin University, Changchun, China;

    Department of Cardiology First Hospital Jilin University No. 71 of the XinMin Street Changchun 130021 Jilin Province China;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

  • 入库时间 2022-08-17 13:17:40

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