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The Methylation Status of the DDX43 Promoter in Chinese Patients with Chronic Myeloid Leukemia

机译:中国慢性粒细胞白血病患者DDX43启动子的甲基化状态

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摘要

Aberrant DNA methylation is a common epigenetic alteration and an important feature in human cancers. The DEAD box polypeptide 43 (DDX43) has been found to be overexpressed in various solid tumors and some hematologic malignancies. In the present study, we investigated the methylation status of the DDX43 promoter in 87 Chinese patients with chronic myeloid leukemia (CML) using real-time quantitative methylation-specific poly-merase chain reaction and examined the DDX43 transcript in 35 patients using real-time quantitative polymerase chain reaction. DDX43 promoter hypomethylation was observed in 22 (25.3%) CML patients. No significant correlation was found between the hypomethylation of the DDX43 promoter with the age, sex, white blood cell counts, hemoglobin concentration, platelet counts, and chromosomal abnormalities of CML patients (p>0.05). The frequency of DDX43 hypomethylation in patients in the chronic phase, in the accelerated phase, and in blast crisis was 23.4% (15/64), 25.0% (2/8), and 33.3% (5/15), respectively (p>0.05). There was a significant correlation between DDX43 hypomethylation and DDX43 transcript (r=0.469, p=0.004). Our data suggest that hypomethylation of the DDX43 promoter may be an early and frequent molecular event in the development of CML in Chinese patients.
机译:DNA甲基化异常是人类癌症中常见的表观遗传学改变,并且是重要特征。已经发现DEAD盒多肽43(DDX43)在各种实体瘤和某些血液系统恶性肿瘤中过表达。在本研究中,我们使用实时定量甲基化特异性多聚酶链反应研究了87位中国慢性髓性白血病(CML)患者中DDX43启动子的甲基化状态,并通过实时检测35例患者中的DDX43转录本定量聚合酶链反应。在22名(25.3%)CML患者中观察到DDX43启动子低甲基化。 DDX43启动子的低甲基化与年龄,性别,白细胞计数,血红蛋白浓度,血小板计数和CML患者的染色体异常之间无显着相关性(p> 0.05)。慢性期,加速期和爆炸危险中DDX43甲基化不足的频率分别为23.4%(15/64),25.0%(2/8)和33.3%(5/15)(p > 0.05)。 DDX43亚甲基化与DDX43转录本之间存在显着相关性(r = 0.469,p = 0.004)。我们的数据表明,DDX43启动子的低甲基化可能是中国患者CML发生中的早期且频繁的分子事件。

著录项

  • 来源
    《Genetic testing and molecular biomarkers》 |2013年第6期|508-511|共4页
  • 作者单位

    Department of Hematology,Affiliated People's Hospital of Jiangsu University,Zhenjiang,People's Republic of China Laboratory Center, Affiliated People's Hospital of Jiangsu University, Zhenjiang, People's Republic of China;

    Laboratory Center, Affiliated People's Hospital of Jiangsu University, Zhenjiang, People's Republic of China;

    Department of Hematology Affiliated People's Hospital of Jiangsu University 8 Dianli Road Zhenjiang 212002 People's Republic of China;

    Laboratory Center, Affiliated People's Hospital of Jiangsu University, Zhenjiang, People's Republic of China;

    Laboratory Center, Affiliated People's Hospital of Jiangsu University, Zhenjiang, People's Republic of China;

    Department of Hematology,Affiliated People's Hospital of Jiangsu University,Zhenjiang,People's Republic of China;

    Department of Hematology, Affiliated People's Hospital of Jiangsu University,Zhenjiang,People's Republic of China;

    Department of Hematology,Affiliated People's Hospital of Jiangsu University,Zhenjiang,People's Republic of China;

    Department of Hematology,Affiliated People's Hospital of Jiangsu University,Zhenjiang,People's Republic of China;

    Laboratory Center, Affiliated People's Hospital of Jiangsu University, Zhenjiang, People's Republic of China;

    Department of Hematology,Affiliated People's Hospital of Jiangsu University,Zhenjiang,People's Republic of China;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

  • 入库时间 2022-08-17 13:17:39

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