Association of E-Cadherin Single-Nucleotide Polymorphisms with the Increased Risk of Breast Cancer: A Study in South Indian Women

摘要

Background: E-cadherin (CDH1) plays an important role in intercellular adhesion, cell signaling, and cellular differentiation. Association of single-nucleotide polymorphisms (SNPs) of CDH1 has been identified in a number of epithelial malignancies; however, studies related to breast cancer are very few. Aim: To investigate the association between CDH1 SNPs and breast cancer risk in south Indian women. Methods: Genotyping of CDH1 functional SNPs (-347G/GA, -160C/A, and +54C/T) was carried out on genomic DNA of blood from breast cancer patients (n = 202) and controls (n = 250) of south Indian origin by PCR-sequencing and PCR-restriction fragment length polymorphism techniques. Haplotype frequencies for multiple loci and the standardized disequilibrium coefficient (D') for pairwise linkage disequilibrium (LD) were assessed by Haploview Software. Results: The frequencies of -160A/A genotypes (p = 0.038) and -160A alleles (p = 0.046) were significantly higher in patients compared to controls. In addition, the frequency of the - 347GA/— 160A/+ 54C haplotype was also significantly elevated in patients (p = 0.0238). Strong LD was observed between -347G/GA and +54C/T loci (D' = 0.44) in patients compared to controls. Conclusion: The CDH1 -160C/A polymorphism may constitute an inheritable risk factor for breast cancer in south Indian women.