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Development and Application of a Rapid and Simple Loop-mediated Isothermal Amplification Method for Food-borne Salmonella Detection

机译：食源性沙门氏菌检测快速简便的环介导等温扩增方法的开发与应用

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摘要

A loop-mediated isothermal amplification (LAMP) method for rapid detection of the food-borne Salmonella strains had been developed and evaluated in this study. The optimal reaction condition was found to be 65℃ for 45 min, with the detection limit as 1 pg DNA/tube and 100 CFU/reaction. Application of LAMP assays was performed on 214 food-borne Salmonella strains using a rapid procedure and easy result confirmation, where the specificity of LAMP and polymerase chain reaction (PCR) assays was 97.7% (209/214) and 91.6% (196/214), respectively; with a 100% specificity for both assays.
机译：本研究开发并评估了一种环介导的等温扩增（LAMP）方法，用于快速检测食源性沙门氏菌菌株。最佳反应条件为65℃45min，检测限为1 pg DNA /管和100 CFU /反应。 LAMP分析的应用是对214株食源性沙门氏菌菌株的快速检测和简便的结果确认，其中LAMP和聚合酶链反应（PCR）分析的特异性分别为97.7％（209/214）和91.6％（196/214） ）， 分别;对两种测定均具有100％的特异性。

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  • 来源
    《Food science and biotechnology》 |2010年第6期|p.1655-1659|共5页
  • 作者

    Xihong Zhao; Li Wang; Jin Chu; Yanmei Li; Yanyan Li; Zhenbo Xu; Lin Li; Mark E. Shirtliff; Xiaowei He; Yao Liu; Jihua Wang; Liansheng Yang;

  • 作者单位

    College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China,Key Laboratory for Green Chemical Process of Ministry of Education, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan 430073, Hubei, China;

    Food Safety Key Laboratory of Guangdong Povince, College of Food Science, South China Agricultural University, Guangzhou, China;

    College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China;

    Guangzhou Medical College, Guangzhou, China;

    State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China;

    College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China,Department of Microbial Pathogenesis, Dental School, University of Maryland, Baltimore, MD 21201, USA;

    College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China;

    Department of Microbial Pathogenesis, Dental School, University of Maryland, Baltimore, MD 21201, USA,Department of Microbiology and Immunology, School of Medicine, University of Maryland, Baltimore, MD, USA;

    College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China;

    Zhongshan Supervision Testing Institute of Quality & Metrology, Zhongshan, China;

    Guangzhou Wondfo Biotechnology Company, Guangzhou, China;

    College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    loop-mediated isothermal amplification (LAMP); salmonella; rapid detection; food-borne pathogen; invA;

    机译：环介导的等温扩增（LAMP）;沙门氏菌;快速检测;食源性病原体;invA;

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