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Nanoparticle corona analysis gives hints for uptake mechanism

机译:纳米电晕分析为吸收机理提供了提示

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摘要

The protein corona, the interface between a nanoparticle and the environment, has an influence on absorption, distribution and cellular downstream effects exerted by the particle. Superparamagnetic iron oxide nanoparticles (SPIONS) are widely used for a plethora of applications. Therefore, the protein corona that formed around a carboxydextran-coated maghemite (Fe_2O_3-DEX) nanoparticle was analyzed. Separate analyses were carried out for the corona in HepG2 cells, in subcellular fractions of HepG2 cells, as well as in the cell culture supernatant. After incubation the protein corona complex was isolated via magnetization and the corona-forming proteins were identified by LC-ESI-QTOF-MS/MS.
机译:蛋白质电晕是纳米粒子与环境之间的界面,会对粒子所产生的吸收,分布和细胞下游效应产生影响。超顺磁性氧化铁纳米粒子(SPIONS)被广泛用于众多应用中。因此,分析了在羧基葡聚糖包被的磁赤铁矿(Fe_2O_3-DEX)纳米颗粒周围形成的蛋白质电晕。对HepG2细胞,HepG2细胞的亚细胞级分以及细胞培养上清液中的电晕进行了单独的分析。温育后,通过磁化分离蛋白质电晕复合物,并通过LC-ESI-QTOF-MS / MS鉴定形成电晕的蛋白质。

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  • 来源
    《Lebensmittelchemie》 |2017年第4期|95-95|共1页
  • 作者单位

    BfR, German Federal Institute for Risk Assessment, Berlin;

    BfR, German Federal Institute for Risk Assessment, Berlin;

    BfR, German Federal Institute for Risk Assessment, Berlin;

    BfR, German Federal Institute for Risk Assessment, Berlin;

    BfR, German Federal Institute for Risk Assessment, Berlin;

    BfR, German Federal Institute for Risk Assessment, Berlin;

    BfR, German Federal Institute for Risk Assessment, Berlin;

    BfR, German Federal Institute for Risk Assessment, Berlin;

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  • 入库时间 2022-08-17 23:27:30

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