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A potential practical approach to reduce Ara h 6 allergenicity by gamma irradiation

机译:一种通过γ射线降低Ara h 6致敏性的潜在实用方法

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摘要

Peanut allergen Ara h 6 was isolated and irradiated at 1, 3, 5, or 10 kGy, and a whole peanut protein extract (WPPE) was also treated by irradiation. Alteration in structure of Ara h 6 was characterised by circular dichroism (CD) spectroscopy, ultraviolet (UV) absorption spectroscopy, fluorescence spectroscopy and SDS-PAGE, and antigenicity was evaluated by immunoblotting and indirect ELISA with anti-Ara h 6 polyclonal antibody. Irradiation induced significant changes in the secondary and tertiary structures of Ara h 6, and the antigenicity of both purified Ara h 6 and WPPE were reduced upon increasing the irradiation doses. Moreover, a good correlation between the loss in ot-helix and IgG binding to Ara h 6 was observed. This indicated that irradiation might be an efficient approach to reduce or eliminate peanut allergenicity.
机译:分离花生过敏原Ara h 6并以1、3、5或10 kGy进行辐照,还通过辐照处理了整个花生蛋白提取物(WPPE)。通过圆二色谱(CD)光谱,紫外(UV)吸收光谱,荧光光谱和SDS-PAGE表征Ara h 6的结构变化,并通过免疫印迹和抗Ara h 6多克隆抗体的间接ELISA评估抗原性。辐照诱导了Ara h 6的二级和三级结构的显着变化,纯化的Ara h 6和WPPE的抗原性均随着辐照剂量的增加而降低。此外,观察到ot-螺旋的损失与IgG与Ara h 6的结合之间具有良好的相关性。这表明辐照可能是减少或消除花生过敏性的有效方法。

著录项

  • 来源
    《Food Chemistry》 |2013年第4期|1141-1147|共7页
  • 作者单位

    State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China,Taizhou Vocational College of Science & Technology, Taizhou 318020, China;

    State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China,Department of Nutrition and Food Hygiene, Anhui Medical University, Hefei 230032, China;

    State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China,Department of Food Science, Nanchang University, Nanchang 330047, China;

    State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China,Department of Food Science, Nanchang University, Nanchang 330047, China;

    State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China,Sino-Cerman joint Research Institute, Nanchang University, Nanchang 330047, China;

    State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China,Sino-Cerman joint Research Institute, Nanchang University, Nanchang 330047, China;

    State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China,Sino-Cerman joint Research Institute, Nanchang University, Nanchang 330047, China;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    gamma irradiation; peanut allergy; ara h 6; protein structure; allergenicity;

    机译:伽马射线花生过敏ara h 6;蛋白质结构;致敏性;

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