Comparison of new approach of GC-HRMS (Q-Orbitrap) to GC-MS/MS (triple-quadrupole) in analyzing the pesticide residues and contaminants in complex food matrices

摘要

Performances of multiresidue analysis of one hundred of pesticides and contaminants, using GC-Q-Orbitrap method in full scan mode were compared to those obtained with GC-triple-quadrupole method in multiple reaction monitoring mode. In terms of sensitivity, 86% of molecules exhibited lower limit of detection values using GC-Q-Orbitrap than using GC-triple-quadrupole. For the GC-Q-Orbitrap method, more than 85% of the pesticides and contaminants showed good recovery [70-120%] in wheat samples, with relative standard deviation values 20%. GC-Q-Orbitrap method appeared the most sensitive for most pesticides studied in wheat with limit of quantification values ranged between 0.1 mu g/kg and 4 mu g/kg. Moreover, the matrix effect was acceptable in wheat extracts for 84 molecules but strong suppression of the chromatographic signal was observed for 16 molecules for the GC-Q-Orbitrap method. The injection of unpurified wheat extracts spiked at 10 mu g/kg proved the potential of the GC-Q-Orbitrap method for use in performing high-throughput pesticide screening.