A convenient and rapid method for detecting D-glucose in honey used smartphone

摘要

Information concerning food composition, including information on its glucose content, is essential for modern food industry due to greater consumer awareness and expectations. In this work, the gene encoding D-glucose dehydrogenase (GDH) from Bacillus Natto was expressed in Escherichia coli BL21(DE3) firstly. Ni-IDA column was used for the purification of GDH. Then, the purified GDH was used to construct a color system with stable and effective measurement of concentration of D-glucose. The smart phone photographing and the software Microsoft Photoshop have been used in the system for determination of the color. The enzymatic analysis system can detect the concentration of D-glucose from 5 mM to 40 mM, and other various sugars has no interference to the system. The system was used to quantitatively detect the concentration of D-glucose in honey. The system can be used for convenient and rapid detection of D-glucose in food, especially for large numbers of samples.